Improved Annotation of the Peach (Prunus persica) Genome and Identification of Tissue- or Development Stage-Specific Alternative Splicing through the Integration of Iso-Seq and RNA-Seq Data
Alternative splicing (AS) is an important way to generate notable regulatory and proteomic complexity in eukaryotes.However, accurate full-length splicing isoform discovery by second-generation sequencing (SGS) technologies is beset with the precise assembly of multiple isoforms from the same gene loci.In recent years, third-generation sequencing (